The ad features footage of Clinton speaking at a Friday fundraiser, where she described the "deplorables" as "racist, sexist, homophobic, xenophobic, Islamaphobic" - you name it."
A narrator says: "You know what's deplorable? Hillary Clinton viciously demonizing hard-working people like you."
Clinton has said she regretted generalizing so broadly.
Trump's campaign says it will spend around $2 million to run the ad this week in the battleground states of Ohio, Pennsylvania, North Carolina and Florida.